Dr. Chris Marquis
- Position: Senior Lecturer
- Room: S130, Samuels
- Phone: (+61 2) 9385 3898
- Fax: (+61 2) 9385 1483
- email: c.marquis@unsw.edu.au
Professional Experience
- 1994-ongoing: Lecturer, UNSW. Senior Lecturer from 2004
Research Contribution
Through our track record in the production of antibodies and recombinant proteins, we were able to secure funding through NCRIS to provide a research infrastructure node in recombinant protein production for the research community. Funding support of $1.75M over 5 years from DEST, the NSW State Government and UNSW supports this initiative. We will have 3 full time staff, fermentation capacity to 25L and a variety of mid and downstream processing capabilities. The Centre's activities are based in E25, which has been renovated and re-organised to accommodate this function.
Over the past 6 years I have had a collaboration with Prof Sam Breit of the Centre for Immunology, St. Vincents Hospital. This has involved generating a process to improve production of recombinant MIC-1 and development of hybridomas producing antibodies to MIC-1 and CLIC-1. We successfully submitted an ARC-Linkage grant for further work on MIC-1 together with Prof Paul Curmi in 2007, obtaining a grant of 514K from the ARC.
Collaborations with Prof Rose Amal (ARC Centre for Functional Nanomaterials) have led to developments of projects in two broad areas. Firstly, the evaluation of the toxicity nanoparticulate catalysts and secondly, the evaluation of nanoparticles in gene delivery to mammalian cells. I co-supervise a PhD student working on this project. We are proposing to develop a new project in the evaluation of nanoparticulate safety using cell culture and newly developed biomolecular tools and have recruited a PhD student to commence work on this project. Further, I am commencing a collaboration with Dr. Volga Bulmus on siRNA delivery to mammalian cells.
Honours & Awards
- Member Ausbiotech Committee NSW
Active Research Projects
- Engineering of a Protein Scaffold for Functional Drug Delivery Applications
- Metabolic Profiling of Gene Induction in Yeast
- Production and Purification of Peanut Allergen Proteins
- Transient Expression of Recombinant Proteins in CHO Cells Based on an Episomal Replication System
Publications
- Huang, E.H., Marquis, C.P. and Gray, P.P. (2007)
- Development of Super-CHO protein free medium based on a statistical design
- Journal of Chemical Technology and Biotechnology 82:431-441
- Marquis, C.P., Baird, A.H., de Nys, R., Holmstrom, C. and Koziumi, N. (2005)
- An evaluation of the antimicrobial properties of the eggs of eleven species of scleractinian corals.
- Coral Reefs 24:248-253
- Coleman, H.M., Marquis, C.P., Scott, J.A., Chin, S.-S, Amal, R. (2005)
- Bactericidal effects of titanium-dioxide-based photocatalysts
- Chemical Engineering Journal, (Environmental Chemical Engineering) 113:55-63
- Huang, E., Marquis, C.P and Gray, P.P (2004)
- Process development for a recombinant Chinese Hamster Ovary Cell Culture with a metal-induced and amplified metallothionine expression system.
- Biotechnology and Bioengineering 88(4):437-450
- M. Toh, M.C. Moffitt, L. Henrichsen, J.M. Cox, C.P. Marquis, P. Mortensen, C.L. Hansen, and B.A. Neilan (2004)
- Cereulide, the emetic toxin of Bacillus cereus, is putatively a product of non-ribosomal peptide synthesis.
- Journal of Applied Microbiology 97(5): 992-1000.
- Toh, M., , Moffitt, M.C., Henrichsen, L., Cox, J.M., Marquis, C.P., Mortensen, P., Hansen, C.L. and Neilan, B.A. (2004)
- Cereulide, the emetic toxin of Bacillus cereus, is putatively a product of non-ribosomal peptide synthesis.
- Journal of Applied Microbiology 97(5): 992-1000.
- Catzel, D., Lalevski, H., van Dyk, D., Gray, P., Marquis C.P. and Mahler, S.M. (2003)
- Purification of human growth hormone using Gradiflow technology.
- Protein Expression and Purification. 32: 126-134