Protein-protein interactions is a component of the rapid development of bioinfomatics within BABS which is being fostered, with others, by Professor Marc Wilkins.
Signalling Pathways Involved in Biofilm Development and Dispersal
Understanding of environmental signals, regulatory mechanisms and the corresponding expression of target genes involved in the biofilm developmental process is crucial for biofilm control strategies. Only recently, the novel, intracellular signaling molecule cyclic diguanosinmonophosphate (c-di-GMP) has been found to play a key role in regulating biofilm development in response to environmental conditions.
The goal of this project is to gain further insights in specific c-di-GMP-dependent processes in the opportunistic pathogen Pseudomonas aeruginosa, using model systems which have been identified and established in our laboratory.
The spectra of methods will cover a wide range of techniques, such as reporter gene expression, random and site-directed mutagenesis, PCR, cloning, bacterial two-hybrid systems, enzymatic tests, virulence assays and competition experiments.
BABS personnel that are responsible for this project