Spoligotype patterns evolve through the deletion of spacer sequences that cannot be recovered and have provided Dr Mark Tanaka with a rich source of data with which to understand the transmission of disease.
Detecting Changes in Protein-Protein Interactions
We know that most proteins inside a cell do not function by themselves: they function as part of protein complexes. However, we have a poor understanding of how complexes can change in their protein composition, and how individual proteins can change their interactions.
We have recently developed new technology for the large-scale purification and analysis of protein complexes. This results in the separation of hundreds of complexes from eukaryotic cells. We can now combine this technology with differential display techniques.
This will give us some of the first glimpses on the dynamics of protein-protein interactions in the cell. We will first trial this process in S. cerevisiae, then apply it to the study of diseased human cells and tissues.
BABS personnel that are responsible for this project