Associate Professor Peter White combines techniques in molecular biology and bioinformatics to describe the evolution of Norovirus outbreak strains.
Transient Expression of Recombinant Proteins in CHO Cells Based on an Episomal Replication System
Subject area and Aims: Transient transfection and recombinant protein expression in mammalian cells
Background: CHO is the most commonly used cell line in commercial production of biopharmaceutical products, the availability of a high-level transient expression system for CHO would be useful since this would allow early product development to be done in the same parental cell line as in a final bioprocess using a stably derived recombinant clone. To improve the level of transient protein expression in CHO, an efficient transient expression system based on episomal replication and plasmid retention in these cells have been developed in our lab.
Significance and current work: Evaluation of an episomal replication system has revealed prolonged expression in serum containing attachment dependent culture. The goals of this project are twofold. Firstly, to demonstrate efficient transient expression in serum-free, suspension culture. Secondly, to attempt to develop an even more efficient host clone for transient expression, utilising flow cytometric cell sorting.
BABS personnel that are responsible for this project