Dominic Glover

Senior Lecturer

Research Program

The intricate and ordered complexes that proteins adopt in nature is central to many biological processes, ranging from cellular scaffolding provided by cytoskeletal proteins to the encapsulation of nucleic acids in viral capsids. Exploiting this remarkable fidelity and precision in self-assembly is highly attractive for the fabrication of structurally defined materials with nanometer dimensions. Advances in the computational prediction of protein folding have enabled the design of proteins that self-assemble into complex yet predictable shapes.

My research applies synthetic biology for the engineering of proteins into structured and functional biomaterials. Central to this approach is the creation of standardised protein building blocks composed of ultrastable filamentous proteins and connectors that assemble into geometrically-defined structures of controllable size and shape. This ability to design protein nanostructures with atomic-level accuracy opens new possibilities in biomaterials. Initial applications of this technology include the creation of electrically conductive protein nanowires for biosensors and enzyme catalysis, the engineering of metabolic pathways, and the fabrication of tissue scaffolds for regenerative medicine.

Current collaborations include Professor Doug Clark (UC Berkeley), A/Professor Allon Hochbaum (UC Irvine), A/Professor Nicholas Stephanopoulos (Biodesign Institute, Arizona State University), and A/Professor Lisa Martin (Monash University).
 

Professional Experience

  • 2017-current: Senior Lecturer, UNSW School of Biotechnology and Biomolecular Sciences
  • 2016: Project Scientist, Department of Chemical and Biomolecular Engineering, University of California, Berkeley, USA
  • 2014-2015: Associate Specialist, Department of Chemical and Biomolecular Engineering, University of California, Berkeley, USA
  • 2010-2014: Postdoctoral Research Fellow, Department of Chemical and Biomolecular Engineering, University of California, Berkeley, USA

 

PUBLICATIONS

Journal articles
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McCluskey JB; Clark DS; Glover DJ, 2020, 'Functional Applications of Nucleic Acid–Protein Hybrid Nanostructures', Trends in Biotechnology, vol. 38, pp. 976 - 989, http://dx.doi.org/10.1016/j.tibtech.2020.02.007
2020
Winter DL; Iranmanesh H; Clark DS; Glover DJ, 2020, 'Design of Tunable Protein Interfaces Controlled by Post-Translational Modifications', ACS synthetic biology, vol. 9, pp. 2132 - 2143, http://dx.doi.org/10.1021/acssynbio.0c00208
2020
Lim S; Clark DS; Glover DJ, 2020, 'Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes', Methods in molecular biology (Clifton, N.J.), vol. 2073, pp. 101 - 115, http://dx.doi.org/10.1007/978-1-4939-9869-2_7
2020
Chen YX; Ing NL; Wang F; Xu D; Sloan NB; Lam NT; Winter DL; Egelman EH; Hochbaum AI; Clark DS; Glover DJ, 2020, 'Structural Determination of a Filamentous Chaperone to Fabricate Electronically Conductive Metalloprotein Nanowires', ACS Nano, vol. 14, pp. 6559 - 6569, http://dx.doi.org/10.1021/acsnano.9b09405
2020
Glover DJ; Xu D; Clark DS, 2019, 'Shaping the Future of Protein Engineering', Biochemistry, vol. 58, pp. 1019 - 1021, http://dx.doi.org/10.1021/acs.biochem.8b01322
2019
Lim S; Jung GA; Glover DJ; Clark DS, 2019, 'Enhanced Enzyme Activity through Scaffolding on Customizable Self-Assembling Protein Filaments', Small, vol. 15, pp. e1805558, http://dx.doi.org/10.1002/smll.201805558
2019
Lim S; Jung GA; Muckom RJ; Glover DJ; Clark DS, 2019, 'Engineering bioorthogonal protein-polymer hybrid hydrogel as a functional protein immobilization platform', Chemical Communications, vol. 55, pp. 806 - 809, http://dx.doi.org/10.1039/C8CC08720B
2019
Lim S; Glover DJ; Clark DS, 2018, 'Prefoldins in archaea', Advances in experimental medicine and biology, vol. 1106, pp. 11 - 23, http://dx.doi.org/10.1007/978-3-030-00737-9_2
2018
Clark DS; Glover DJ, 2018, 'Controlled assembly of the filamentous chaperone gamma-prefoldin into defined nanostructures', Methods in Molecular Biology, vol. 1798, pp. 293 - 306, http://dx.doi.org/10.1007/978-1-4939-7893-9_22
2018
Glover DJ; Lim S; Xu D; Sloan NB; Zhang Y; Clark DS, 2018, 'Assembly of Multicomponent Protein Filaments Using Engineered Subunit Interfaces', ACS Synthetic Biology, vol. 7, pp. 2447 - 2456, http://dx.doi.org/10.1021/acssynbio.8b00241
2018
Glover DJ; Clark DS, 2016, 'Protein calligraphy: A new concept begins to take shape', ACS Central Science, vol. 2, pp. 438 - 444, http://dx.doi.org/10.1021/acscentsci.6b00067
2016
Rowe CL; Wagstaff KM; Oksayan S; Glover DJ; Jans D; Moseley GW, 2016, 'Nuclear trafficking of the rabies virus interferon antagonist p-protein is regulated by an importin-binding nuclear localization sequence in the C-Terminal domain', PLoS ONE, vol. 11, http://dx.doi.org/10.1371/journal.pone.0150477
2016
Glover DJ; Giger L; Kim SS; Naik RR; Clark DS, 2016, 'Geometrical assembly of ultrastable protein templates for nanomaterials', Nature Communications, vol. 7, http://dx.doi.org/10.1038/ncomms11771
2016
Glover DJ; Clark DS, 2015, 'Oligomeric assembly is required for chaperone activity of the filamentous γ-prefoldin', FEBS Journal, vol. 282, pp. 2985 - 2997, http://dx.doi.org/10.1111/febs.13341
2015
Glover DJ; Giger L; Kim JR; Clark DS, 2013, 'Engineering protein filaments with enhanced thermostability for nanomaterials', Biotechnology Journal, vol. 8, pp. 228 - 236, http://dx.doi.org/10.1002/biot.201200009
2013
Glover DJ, 2012, 'Artificial viruses: Exploiting viral trafficking for therapeutics', Infectious Disorders - Drug Targets, vol. 12, pp. 68 - 80, http://dx.doi.org/10.2174/187152612798995000
2012
Glover DJ; Leyton DL; Moseley GW; Jans DA, 2010, 'The efficiency of nuclear plasmid DNA delivery is a critical determinant of transgene expression at the single cell level', Journal of Gene Medicine, vol. 12, pp. 77 - 85, http://dx.doi.org/10.1002/jgm.1406
2010
Moseley GW; Leyton DL; Glover DJ; Filmer RP; Jans DA, 2010, 'Enhancement of protein transduction-mediated nuclear delivery by interaction with dynein/microtubules', Journal of Biotechnology, vol. 145, pp. 222 - 225, http://dx.doi.org/10.1016/j.jbiotec.2009.11.015
2010
Kaur G; Delluc-Clavieres A; Poon IKH; Forwood JK; Glover DJ; Jans DA, 2010, 'Calmodulin-dependent nuclear import of HMG-box family nuclear factors: Importance of the role of SRY in sex reversal', Biochemical Journal, vol. 430, pp. 39 - 48, http://dx.doi.org/10.1042/BJ20091758
2010
Glover DJ; Su MN; Mechler A; Martin LL; Jans DA, 2009, 'Multifunctional protein nanocarriers for targeted nuclear gene delivery in nondividing cells', FASEB Journal, vol. 23, pp. 2996 - 3006, http://dx.doi.org/10.1096/fj.09-131425
2009
Roth DM; Moseley GW; Glover D; Pouton CW; Jans DA, 2007, 'A microtubule-facilitated nuclear import pathway for cancer regulatory proteins', Traffic, vol. 8, pp. 673 - 686, http://dx.doi.org/10.1111/j.1600-0854.2007.00564.x
2007
Wagstaff KM; Glover DJ; Tremethick DJ; Jans DA, 2007, 'Histone-mediated transduction as an efficient means for gene delivery', Molecular Therapy, vol. 15, pp. 721 - 731, http://dx.doi.org/10.1038/sj.mt.6300093
2007
Glover D; Glouchkova L; Lipps H; Jans DA, 2007, 'Overcoming cellular barriers to safe, sustained and efficient non-viral gene therapy', Advances in Gene and Molecular Cell Therapy, vol. 1, pp. 126 - 140
2007
Glover DJ; Lipps HJ; Jans DA, 2005, 'Towards safe, non-viral therapeutic gene expression in humans', Nature Reviews Genetics, vol. 6, pp. 299 - 310, http://dx.doi.org/10.1038/nrg1577
2005
Glover D; Little JB; Lavin MF; Gueven N, 2003, 'Low dose ionizing radiation‐induced activation of connexin 43 expression', International Journal of Radiation Biology, vol. 79, pp. 955 - 964, http://dx.doi.org/10.1080/09553000310001632895
2003
Conference Papers
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Lim S; Glover D; Clark D, 2017, 'Modular design and functionalization of enzymatic hydrogels using a self-assembling protein building block', in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, presented at American Chemical Society meeting
2017
Glover D; Giger L; Reese H; Clark DS, 2014, 'Engineering the self-assembly of ultrastable protein filaments into 2D and 3D multifunctional nanostructures', in Nanoscale Science and Engineering Forum 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future, pp. 44
2014