Seahorse Extracellular Flux (XF) Analyser

The Seahorse Extracellular Flux (XF) Analyser uses new technology for cellular bioenergetic studies. The Seahorse measures the metabolic activity of cells in minutes, offering a physiologic cell-based assay for the determination of basal oxygen consumption, glycolysis, ATP turnover and respiratory capacity in a single experiment. The two major energy producing pathways of the cell, mitochondrial respiration and glycolysis, are measured simultaneously and the data generated provides the most physiologically relevant bioenergetic assay available for the determination of mitochondrial function.

Adherent cells, primary cells, suspension cells and isolated mitochondria can all be analysed, allowing measurement of mitochondrial function and dysfunction in living cells in real-time.
The system can be used to study respiratory malfunction in multiple diseases including cancer, cardiovascular disease, ageing-associated disorders, mitochondrial diseases, immunological disorders, neurodegenerative disease, obesity and diabetes. The Seahorse is useful to anyone studying mitochondrial abnormality as a consequence of environmental insult, mitochondrial DNA or nuclear DNA mutation. Assays are non-invasive, allowing for further downstream applications to be performed.

RA list of publications on a variety of disorders studied are provided on the Seahorse website:


The Seahorse can measure 24 wells of culture or isolated mitochondria in under 2 hours. The cost to external users is as follows:

  • Level 1 = $200 per plate. This is the flat rate charge. Users will supply their own plates and all reagents and operate the machine after training. 
  • Level 2 = $300 per plate. BABS will supply the plates but the user will supply all the reagents for the assay and operate the machine after training.
  • Level 3 = $600 per plate. BABS will supply the plates and all the reagents but the user will perform their own assays and operate the machine after training.

BABS Contact

All inquiries should be directed to Professor Bill Ballard
Phone:  (02) 9385-2021