New Developments in Biological TEM, An Emerging Epoch

Speaker: 
Professor Paul Matsudaira
Affiliation: 
Dept of Biological Sciences, NUS Centre for BioImaging Sciences, MechanoBiology Institute, Singapore
Date: 
8 October 2012 - 12:00pm
Venue: 
Rountree Room 356, Biological Sciences Building
Abstract: 

In five years time, biological TEM will be vastly different because of new technologies and methods, including: microfabricated liquid cells for imaging protein in liquid water, phase contrast TEM, and CMOS cameras. The liquid cells are the size of a TEM grid, but able to contain liquid samples that are sealed against the vacuum of the TEM. Although the phase contrast is standard for the light microscope, the technologies for generating phase contrast in a TEM are only now emerging. A simple carbon film is the basis for the first generation phase contrast TEMs. Finally, images are routinely recorded on film or by CCD.

However, these two devices will be replaced with a CMOS camera because it can directly record images, and at a resolution comparable to film. With these new developments, the difference will be seen in studying dynamic processes in real-time and molecular structures solved at significantly higher resolution. Examples will be taken from our work on imaging water and protein in water.

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